Device

Part:BBa_K1465304:Design

Designed by: iGEM-Team Bielefeld 2014   Group: iGEM14_Bielefeld-CeBiTec   (2014-10-08)


adhA gene from Lactococcus lactis under T7 control


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 467
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Classical Suffix Insertion of the adhA gene from Lactococcus lactis (BBa_K1465301) into the pSB1A2 backbone of the T7 promoter (BBa_I719005). Afterwards successful recloning in pSB1C3 which lead to this part (pSB1C3_T7_adhA)

Source